|
KMID : 0545120010110030423
|
|
Journal of Microbiology and Biotechnology
2001 Volume.11 No. 3 p.423 ~ p.427
|
|
|
Effect of Collagen Concentration on the Viability and Metabolic Function of Encapsulated Hepatocytes
|
|
Kim, Sung Koo
Yu, Sun Hee/Lee, Ji Hyun/Lee, Ji Young/Rademacher, Axel/Lee, Doo Hoon/Park, Jung Keug
|
|
|
Abstract
|
|
|
|
Chitosan/alginate capsules were formed by electrostatic interactions and had appropriate mechanical strength, permeability to albumin, and stability to hepatocytes. Rat hepatocytes were isolated and immobilized in chitosan/alginate capsules. During the encapsulation process with hepatocyte, 10% of viability was decreased mainly due to the low pH of the chitosan solution. Among various capsule fabrication methods, the chitosan-alginate capsule showed the highest mechanical strength. Addition of collagen in the capsule with hepatocytes enhanced hepatic metabolism as well as the cell viability for 2 weeks of culture. The hepatocyte in the capsule without collagen decreased the viability to 10% for 2-week cultures.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|
|